Coding

Part:BBa_K3726025

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-05)


CDS_GarK (E. Coli BL21)

This part corresponds with the protein coding sequence for a Glycerate 2-kinase

The codified enzyme from Escherichia coli (strain BL21) catalyzes the phosphorylation of (R) - glycerate to transform it into (2R) -2-phosphoglycerate. ATP dependent.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 355
    Illegal AgeI site found at 868
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None